The Gastrointestinal Microbial Assay Plus (GI-MAP)- Comprehensive panel of microbial targets, immune and digestive markers The Gastrointestinal Microbial Assay Plus (GI-MAP) is an innovative clinical tool that measures gastrointestinal microbiota DNA from a single stool sample with state of the art, quantitative polymerase chain reaction (qPCR) technology. The GI-MAP was designed to detect microbes that may be disturbing normal microbial balance or contributing to illness as well as indicators of digestion, absorption, inflammation, and immune function. With a culture based test it is not possible to measure strict anaerobes, viruses or virulence factors. The GI-MAP's accuracy and reliability allows personalized protocols to address gut dysfunction based on which infections are urgent, which areas of the gut are already optimized, and which areas should be addressed after an infection is resolved. Additionally, the quantification of identified micro-organisms offers a remarkable ability to see how treatment modalities are working because a retest after treatment can show whether a parasite has resolved, dysbiosis has improved, and more. Learn more about the GI-MAP in clinical practice by watching our webinar: https://www.youtube.com/watch?v=QL_ekGVOdaE Indications - Autoimmune molecular mimicry (if caused by pathogens)
- Diarrhea
- Digestion
- Foodborne illness
- Gastritis
- Gastroenteritis (if it has viral causes)
- Gut dysbiosis
- Immunity
- Inflammation
- Suspected bacterial, parasitic or viral pathogenesis (incl. detection of antibiotic resistant bacteria)
Overview The Gastrointestinal Microbial Assay Plus (GI-MAP) was designed to assess a patient’s microbiome from a single stool sample, with particular attention to microbes that may be disturbing normal microbial balance and may contribute to perturbations in the gastrointestinal (GI) flora or illness. The panel is a comprehensive collection of microbial targets as well as immune and digestive markers. It screens for pathogenic bacteria, commensal bacteria, opportunistic pathogens, fungi, viruses, and parasites. It primarily uses multiplex, automated, DNA analysis to give integrative and functional medicine practitioners a better view into the gastrointestinal microbiome. Methodology Quantitative polymerase chain reaction (qPCR) Analytes BACTERIAL PATHOGENS - Campylobacter
- C. difficile Toxin A
- C. difficile Toxin B
- Enterohemorrhagic E. coli
- E. coli O157
- Enteroinvasive E. coli/Shigella
- Enterotoxigenic E. coli LT/ST
- Shiga-like Toxin E. coli stx1
- Shiga-like Toxin E. coli stx2
- Salmonella
- Vibro cholerae
- Yersinia enterocolitica
- H. pylori
Virulence Factor, babA Virulence Factor, cagA Virulence Factor, dupA Virulence Factor, iceA Virulence Factor, oipA Virulence Factor, vacA Virulence Factor, virB Virulence Factor, virD
PARASITIC PATHOGENS - Cryptosporidium
- Entamoeba histolytica
- Giardia
VIRAL PATHOGENS - Adrenovirus 40/41
- Norovirus GI
- Norovirus GII
NORMAL/COMMENSAL BACTERIA - Akkermansia Mucinophilia
- Bacteroides fragilis
- Bifidobacterium spp.
- Clostridia (class)
- Enterobacter spp.
- Enterococcus spp.
- Escherichia spp.
- Faecalbacterium prausnitzii
- Lactobacillus spp.
BACTERIAL PHYLA - Bacteroidetes
- Firmicutes
- Firmicutes/Bacteroidetes Ratio
ADDITIONAL DYSBIOTIC/OVERGROWTH BACTERIA - Bacillus spp.
- Enterococcus faecalis
- Enterococus faecium
- Morganella spp.
- Pseudomonas spp.
- Pseudomonas aeruginosa
- Staphylococcus spp.
- Staphylococcus aeureus
- Streptococcus spp.
- Methanobacteriaceae (family)
POTENTIAL AUTOIMMUNE TRIGGERS - Citrobacter spp.
- Citrobacter freundii
- Fusobacterium spp.
- Klebsiella spp.
- Klebsiella pneumoniae
- Mycobacterium avium subsp. paratuberculosis
- Prevotella spp
- Proteus spp.
- Proteus mirabilis
FUNGI/YEAST - Candida albicans
- Candida spp.
- Geotricum spp.
- Microsporidia spp.
- Rhodoturula spp.
OPPORTUNISTIC VIRUSES - CMV- Cytomegalovirus
- EBV- Epstein Bar Virus
PARASITES & WORMS: PROTOZOA - Blastocystis hominis
- Chilomastix mesnelli
- Cyclospora spp.
- Dientamoeba fragilis
- Endolimax nana
- Entamoeba coli
- Pentatrichomonas hominis
WORMS - Ancyclostroma duodenale
- Ascaris lumbricoides
- Necator americanis
- Trichuris trichiura
- Taenia spp
INTESTINAL HEALTH MARKERS: DIGESTION IMMUNE RESPONSE INFLAMMATION GI MARKERS - ß-Glucuronidase
- Occult Blood - FIT
ANTIBIOTIC RESISTANCE GENES (Optional add-on): Phenotypes | HELICOBACTER - Amoxicillen
- Clarithromycin
- Fluroquinolines
- Tetracycline
Specimen Stool sample Container - 1x orange-capped stool vial
Patient preparation - Please refrain from taking aspirin for two days prior to collecting your sample.
- Never discontinue prescription medication without first consulting your physician.
Age requirements Can be used on all ages. However, often the microbiome of infants will appear very “dysbiotic”, as the microbiome and the infant’s immune system is very immature. Available add-ons: • Zonulin Medications We recommend continuing all prescription medications prior to testing. Please carefully document any relevant recent or current medication use. Medications that may alter the composition of the microbiome include: - Antibiotics -(after finishing Antibiotics, wait 4-6 weeksbefore collecting sample)
- Immune Suppressants/ Oral steroids- will likely cause a lower Secretory IgA, Anti-gliadin, and calprotectin result (after finishing these meds, wait 4-6 weeks before collecting sample)
Supplements:
It is not necessary to discontinue most supplements before testing. It is the provider’s discretion to recommend discontinuing supplements. Many providers will want to understand the impact of supplements on the gut ecosystem and will want their patients to continue supplements during testing. If a provider wants more of a “baseline” assessment using the GI-MAP, they may recommend discontinuing supplements prior to testing. There are a few categories of supplements that deserve consideration:
Non-prescription anti-microbial agents:
While we don’t require that people discontinue antibiotics or antimicrobial substances before collection their stool sample, doing the test while taking these substances can influence the test results. If possible, we recommend discontinuing anti-microbial agents 4-6 weeks before testing.
Enzymes:
Oral enzyme use will not change the elastase-1 finding. If the enzyme contains lipase or ox bile, it may decrease the value for steatocrit.
Ox Bile/Lipase:
Ox bile/lipase intake will affect the steatocrit level but will not affect Elastase-1. If you would like to see your patient’s baseline without supplementation, discontinue at least 3 days before testing. If you would like to see how well-managed your patient is on their supplement, do not discontinue.
Probiotics:
Probiotic supplements can influence the normal and opportunistic bacteria flora. If you would like to assess your patient’s baseline microbial balance without supplementation, discontinue for ~2 weeks prior to testing. If you would like to see the influence of the current probiotic supplement, do not discontinue prior to testing.
Biofilm disruptors:
There is no published evidence to suggest that a biofilm disruptor will improve detection of organisms. However, some clinicians choose to use a biofilm disruptor with their patients up to 14 days before testing. This is an option but not a requirement for testing.
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See also Brochure under ‘Downloadable files’ for even more research.
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